A service-oriented Grid environment with on-demand QoS support

Grid Computing entstand aus der Vision für eine neuartige Recheninfrastruktur, welche darauf abzielt, Rechenkapazität so einfach wie Elektrizität im Stromnetz (power grid) verfügbar zu machen. Der entsprechende Zugriff auf global verteilte Rechenressourcen versetzt Forscher rund um den Globus in die Lage, neuartige Herausforderungen aus Wissenschaft und Technik in beispiellosem Ausmaß in Angriff zu nehmen. Die rasanten Entwicklungen im Grid Computing begünstigten auch Standardisierungsprozesse in Richtung Harmonisierung durch Service-orientierte Architekturen und die Anwendung kommerzieller Web Services Technologien. In diesem Kontext ist auch die Sicherung von Qualität bzw. entsprechende Vereinbarungen über die Qualität eines Services (QoS) wichtig, da diese vor allem für komplexe Anwendungen aus sensitiven Bereichen, wie der Medizin, unumgänglich sind. Diese Dissertation versucht zur Entwicklung im Grid Computing beizutragen, indem eine Grid Umgebung mit Unterstützung für QoS vorgestellt wird. Die vorgeschlagene Grid Umgebung beinhaltet eine sichere Service-orientierte Infrastruktur, welche auf Web Services Technologien basiert, sowie bedarfsorientiert und automatisiert HPC Anwendungen als Grid Services bereitstellen kann. Die Grid Umgebung zielt auf eine kommerzielle Nutzung ab und unterstützt ein durch den Benutzer initiiertes, fallweises und dynamisches Verhandeln von Serviceverträgen (SLAs). Das Design der QoS Unterstützung ist generisch, jedoch berücksichtigt die Implementierung besonders die Anforderungen von rechenintensiven und zeitkritischen parallelen Anwendungen, bzw. Garantien f¨ur deren Ausführungszeit und Preis. Daher ist die QoS Unterstützung auf Reservierung, anwendungsspezifische Abschätzung und Preisfestsetzung von Ressourcen angewiesen. Eine entsprechende Evaluation demonstriert die Möglichkeiten und das rationale Verhalten der QoS Infrastruktur. Die Grid Infrastruktur und insbesondere die QoS Unterstützung wurde in Forschungs- und Entwicklungsprojekten der EU eingesetzt, welche verschiedene Anwendungen aus dem medizinischen und bio-medizinischen Bereich als Services zur Verfügung stellen. Die EU Projekte GEMSS und Aneurist befassen sich mit fortschrittlichen HPC Anwendungen und global verteilten Daten aus dem Gesundheitsbereich, welche durch Virtualisierungstechniken als Services angeboten werden. Die Benutzung von Gridtechnologie als Basistechnologie im Gesundheitswesen ermöglicht Forschern und Ärzten die Nutzung von Grid Services in deren Arbeitsumfeld, welche letzten Endes zu einer Verbesserung der medizinischen Versorgung führt.Grid computing emerged as a vision for a new computing infrastructure that aims to make computing resources available as easily as electric power through the power grid. Enabling seamless access to globally distributed IT resources allows dispersed users to tackle large-scale problems in science and engineering in unprecedented ways. The rapid development of Grid computing also encouraged standardization, which led to the adoption of a service-oriented paradigm and an increasing use of commercial Web services technologies. Along these lines, service-level agreements and Quality of Service are essential characteristics of the Grid and specifically mandatory for Grid-enabling complex applications from certain domains such as the health sector. This PhD thesis aims to contribute to the development of Grid technologies by proposing a Grid environment with support for Quality of Service. The proposed environment comprises a secure service-oriented Grid infrastructure based on standard Web services technologies which enables the on-demand provision of native HPC applications as Grid services in an automated way and subject to user-defined QoS constraints. The Grid environment adopts a business-oriented approach and supports a client-driven dynamic negotiation of service-level agreements on a case-by-case basis. Although the design of the QoS support is generic, the implementation emphasizes the specific requirements of compute-intensive and time-critical parallel applications, which necessitate on-demand QoS guarantees such as execution time limits and price constraints. Therefore, the QoS infrastructure relies on advance resource reservation, application-specific resource capacity estimation, and resource pricing. An experimental evaluation demonstrates the capabilities and rational behavior of the QoS infrastructure. The presented Grid infrastructure and in particular the QoS support has been successfully applied and demonstrated in EU projects for various applications from the medical and bio-medical domains. The EU projects GEMSS and Aneurist are concerned with advanced e-health applications and globally distributed data sources, which are virtualized by Grid services. Using Grid technology as enabling technology in the health domain allows medical practitioners and researchers to utilize Grid services in their clinical environment which ultimately results in improved healthcare

On optimising FAC(M) counter missile tactics : a dynamic simulation model to optimise soft kill tactics employed by a generic fast attack craft against a generic surface-to-surface, fire-and-forget missile

The aim of this dissertation is to show how counter missile tactics for a fast attack craft armed with missiles [FAC(M)] against a surface-to-surface, fireand- forget missile [SSM] can be optimised. As a result the ship and missile will be modelled as generic concepts while the environment will be a chosen area of operations. The applicable methodology is to simulate the ship, missile and environment as well as the interactions between them. At the same time, the ship will be carrying out combinations of five separate missile counter measures. The methodology is then to build a dynamic simulation model to optimise soft kill tactics by a generic F AC(M) against a generic SSM in the chosen environment and evaluate the outcome of the simulation by viewing the experiment as a 25 factorial design and to analyse it accordingly.Operations ResearchOperations ManagementM.Sc. (Operations Research

Lasers for LISA: Overview and phase characteristics

We have investigated two alternative laser systems for the Laser Interferometer Space Antenna (LISA). One consisted of the laser of LISA's technology precursor LISA Pathfinder and a fiber amplifier originally designed for a laser communication terminal onboard TerraSar-X. The other consisted of a commercial fiber distributed feedback (DFB) laser seeding a fiber amplifier. We have shown that the TerraSar-X amplifier can emit more than 1W without the onset of stimulated Brillouin scattering as required by LISA. We have measured power noise and frequency noise of the LISA Pathfinder laser (LPL) and the fiber laser. The fiber laser shows comparable or even lower power noise than the LPL. LISA will use electro-optical modulators (EOMs) between seed laser and amplifier for clock noise comparison between spacecraft. This scheme requires that the excess noise added by the amplifiers be negligible. We have investigated the phase characteristics of two fiber amplifiers emitting 1 W and found them to be compatible with the LISA requirement on amplifier differential phase noise.DLR/50 OQ 0501DLR/50 OQ 060

Fiber modulators and fiber amplifiers for LISA

We present the sideband phase characteristics of a fiber-coupled integrated electro-optical modulator (EOM) at a modulation frequency of 2 GHz for Fourier frequencies from 0.1 mHz to 1 Hz. The upper phase noise limit was almost an order of magnitude better than required for LISA. The EOM's phase dependencies on temperature and transmitted optical power were measured and found to be uncritical. Additionally we have investigated three optical amplifiers emitting 1 W. Their differential phase noise and optical pathlength noise as one contribution to differential phase noise were measured. The measured differential phase noise was within the requirement. The dependencies of differential phase noise on pump power were measured and requirements for the operation of the amplifier on the LISA satellite derived.DLR/50 OQ 0601DFG/EXC/QUES

Impacts of 1.5°C Global Warming on Natural and Human Systems

An IPCC Special Report on the impacts of global warming of 1.5°C above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate povert

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century